p53 mediates target gene association with nuclear speckles for amplified RNA expression

Alexander KA, Coté A, Nguyen SC, Zang L, Gholamalamdari O, Agudelo-Garcia P, Lin-Shiao E, Tanim KM, Lim J, Biddle N, Dunagin MC, Good CR, Mendoza MR, Little SC, Belmont A, Joyce EF, Raj A, Berger SL. p53 mediates target gene association with nuclear speckles for amplified RNA expression. Molecular Cell 2021.

This publication demonstrates that a transcription factor, p53, regulates position of chromatin relative to nuclear speckles. The field had known for decades that gene position at speckles is regulatable, however what dictates this positioning was not clear. This publication revealed that gene-speckle targeting by p53 requires intact DNA binding, as well as the p53 proline-rich domain (which we now refer to as the speckle targeting motif), but does not require p53 transactivation functions.